Isotope Dilution Mass Spectrometry: Principles and Applications
Isotope Dilution Mass Spectrometry play an important role in producing LC-MS data that can be interpreted with greater confidence. This article explains the concept in practical terms and connects it with the broader requirements of metabolomics, quantitative analysis, and reproducibility. What Is Isotope Dilution Mass Spectrometry? Isotope dilution mass spectrometry uses an isotopically enriched version of a compound as a reference for measuring the corresponding natural-abundance analyte. The labeled material is distinguishable by mass, allowing the analytical relationship between sample and standard to be evaluated directly. The Core Principle A known amount of labeled material is introduced into the analytical...