1. IROA Internal Standard (IROA-IS)
95% U-13C Labeled Yeast Extract
- 3 vials of lyophilized S. cerevisiae yeast extract
- Unique 95% U-13C labeling
- Compatible with diverse biological samples
- Supports accurate metabolite quantitation
IROA Internal Standard KIT Accurate Metabolite Analysis & Quantitation
Reliable metabolite identification, suppression correction, and sample normalization using a unique 95% U-13C internal standard.
- Identifies and quantifies 400–600 metabolites
- Suppression-corrected measurements
- Universal sample normalization
- Compatible with major LC-MS platforms
400–600
Metabolites Identified
Per sample set analyzed
90
Samples Per Kit
High-throughput workflow capacity
$100
Per 90 Samples
Economical metabolomics standard solution
95%
U-13C Labeling
Unique fully labeled yeast extract
Vendor
Agnostic
Compatible with major LC-MS platforms
Why IROA Internal Standard?
The IROA Internal Standard (IROA-IS) is a fully labeled 95% U-13C yeast extract designed to improve confidence in metabolomics experiments. It enables accurate metabolite identification, suppression correction, and normalization while reducing variability between samples and analytical runs.
Accurate Metabolite Identification
The predictable isotope pattern generated by the IROA-IS allows ClusterFinder software to automatically identify metabolites, fragments, and adducts with high confidence.
Improved Quantitation
Suppression correction algorithms compensate for ionization losses, producing more accurate and biologically meaningful quantitative measurements.
Universal Normalization
Dual MSTUS normalization enables direct comparison of samples across instruments, studies, and time points by normalizing against a true internal standard.
The result is reproducible, comparable, and quantitative metabolomics data that can be trusted across experiments and laboratories.
How the IROA Internal Standard Works
1
Spike into Samples
Add the IROA Internal Standard to experimental samples including blood, tissues, cells, plant material, and other biological matrices.
2
Analyze by LC-MS
The characteristic isotope patterns are automatically detected and aligned using ClusterFinder software.
3
Identify & Quantitate
ClusterFinder identifies metabolites, performs suppression correction, normalizes samples, and generates quantitative results.
WHAT'S INCLUDED
2. ClusterFinder Software
Advanced Analysis Platform
- Automated metabolite identification
- Quantitative analysis tools
- Ion suppression correction
- Sample normalization workflows
3. IROA Metabolite Library
Reference Compound Database
- RT and m/z reference data
- Fragment and adduct information
- Physical compound characteristics
- Editable database support
4. User Manual
Complete Workflow Guide
- Installation instructions
- Workflow setup guidance
- Best practices
- Troubleshooting assistance
Summary of Benefits
Daily instrument QA/QC
Economical $200 for 90 samples
No false data; removal of artifactual peaks
Accurate compound formula ID for MS alone
Corrects for ionization losses for better quantitation
Normalizes samples to a universal standard
Yeast metabolites characterized by multiple LC-MS methods
ClusterFinder builds libraries, IDs and quantitates
Mass-spec vendor agnostic support
Supports biomarker discovery
Helpful Resources
Routinely Identified Compounds in Internal Standard
DownloadIROA Internal Standard Product Sheet
DownloadHow to Set Up and Run a C13-Labeled Yeast Extract TruQuant Calibration Experiment
DownloadNormalization Strategies for Metabonomic Analysis of Urine Samples
DownloadReady to Improve Quantitative Accuracy?
TruQuant delivers reliable identification, ion suppression correction and normalization to elevate your metabolomics research.