Targeted metabolic analyses are very useful and generally more quantitative than non-targeted analyses, but often miss the appropriate compounds. Non-targeted analyses theoretically avoid this problem but their ability to correctly identify peaks and quantify them is a major challenge. The TruQuant protocol was developed as a targeted analysis
for hundreds of compounds in a biochemically-complex Internal Standard (IROA-IS) that was quantitatively enhanced by providing a mechanism for the correction of ion-suppression, and a very advanced sample-to sample normalization. This report concerns the identification of hundreds of additional authenticated peaks that can also be normalized even though they will not be associated with an internal standard.
Amy L. Lane1, Felice de Jong2, Chris Beecher2