Products to Make Metabolic Profiling Easier

Mass Spectrometry Metabolite Library of Standards (MSMLSTM) supplied with software tool MSMLSDiscoveryTM

Take the hassle out of sourcing, preparing compounds and building a library!  MSMLS contains over 600 high quality primary metabolite standards provided in convenient 96 well format. The library of standards are most commonly used to provide retention times and spectra for key metabolic compounds, help optimize mass spectrometry analytical protocols, and qualify and quantify mass spectrometry sensitivity and limit of detection.  MSMLSDiscovery is a software tool designed to streamline and simplify the tedious process of building authentic standard libraries for mass spectrometry and supports the extraction, manipulation, and stoarge of the date generated when using MSMLS.

IROA Biochemical Quantitation Kit Catalog #300-250 (for Mammalian cells)

Like SILAC, IROA utilizes full metabolic labeling to distinguish between two mammalian cell populations but uses both 5% 13C (experimental samples) and 95% 13C (control samples) to remove artifacts/noise, calculate carbon number and molecular formula  to enable metabolite identification.
 

 

News & Events

Workshops

2015 SECIM Workshop: May 11-14, 2015

The 2nd Annual SECIM Metabolomics Workshop will be held Monday, May 11 through Thursday, May 14, 2015, in Gainesville, Florida.

This year’s workshop focuses on metabolomics data processing, data analysis, and metabolite identification, including hands-on training sessions. Attendees are required to bring their own laptops in order to conduct hands-on data analysis. A quick overview of metabolomics, and an introduction to the analytical instrumentation will also be covered.

Conferences

We hope you can join IROA Technologies at the 63rd ASMS Conference, May 31st-June 4th in St Louis

POSTER SESSIONS

MP 373: An Optimized Method for Extraction of Red Blood Cell Metabolites; Mike Williams1 ; Ann Guggisberg2 ; Christopher Beecher1 ; Audrey Odom2 ; Timothy Garrett1 ; 1 University of Florida, Gainesville, Florida; 2 Washington University School of Medicine, St. Louis,
 
MP390: Metabolic Effect of Drought Stress during the Grain Filling Growth Stage in Wheat Measured by Isotopic Ratio Outlier Analysis (IROA); Felice De Jong1 ; Chris Beecher2 ; 1 IROA Technologies LLC, Bolton, MA; 2 University of Florida, Gainesville, FL

TP 153: Novel Strategies for Analysis of High-Resolution DataIndependent MS/MS Spectra for Rapid and Accurate Structural Confirmation of Lipids; Jeremy Koelmel1 ; John Bowden2 ; Candice Ulmer1 ; Rainey Patterson1 ; Timothy Garrett1 ; Chris Beecher1 ; Richard A. Yost1 ; 1 University of Florida, Gainesville, Fl; 2 NIST, Charleston, SC

TP 187: Structural Elucidation of the Metabolome using Isotopic Ratio Outlier Analysis (IROA) in combination with UHPLC-QTOF and Data-Independent Acquisition; Chris Beecher1 ; Felice de Jong1 ; Giuseppe Astarita2 ; 1 IROA Technologies, Ann Arbor, MI; 2 Waters Corporation, Milford, MA

TP 188: Untargeted Metabolomic Analysis of the Yeast Lipin Phosphatidate Phosphatase (Pah1p) Deletion Using Isotopic Ratio Outlier Analysis (IROA) and LC-HRMS; Yu-Hsuan Tsai1 ; Timothy Garrett2 ; Yunping Qiu3 ; Robyn Moir4 ; Ian Willis4 ; Chris Beecher5 ; Richard Yost1, 2; Irwin Kurland3 ; 1 Dept. of Chemistry, Univ. of Florida, Gainesville, Fl; 2 Dept. of Pathology, Univ. of Florida, Gainesville, Fl; 3 Dept. of Medicine, Albert Einstein College of, Bronx, NY; 4 Dept. of Biochemistry, Albert Einstein College, Bronx, NY; 5 IROA Technologies, Ann Arbor, MI

How do Worms Respond to Stress?

SECIM investigators teamed up with industry partner IROA Technologies to conduct isotopic ratio outlier analysis (IROA). In this experiment, we used IROA to “label” or culture two biological samples with different percentages of 13C: a control group of worms, and a group of worms that was gently heated (a heat shock). This labeling technique allowed us to mix together and then analyze differences between the samples. We discovered more than 500 peaks that significantly changed following a heat shock. Now the mystery is to find out the identities of more of these peaks!