Make Profiling Easier
Mass Spectrometry Metabolite Library of Standards (MSMLSTM) supplied with software tool MSMLSDiscoveryTM
Take the hassle out of sourcing, preparing compounds and building a library! MSMLS contains over 600 high quality primary metabolite standards provided in convenient 96 well format. The library of standards are most commonly used to provide retention times and spectra for key metabolic compounds, help optimize mass spectrometry analytical protocols, and qualify and quantify mass spectrometry sensitivity and limit of detection. MSMLSDiscovery is a software tool designed to streamline and simplify the tedious process of building authentic standard libraries for mass spectrometry and supports the extraction, manipulation, and stoarge of the date generated when using MSMLS.
IROA Biochemical Quantitation Kit Catalog #300-250 (for Mammalian cells)
Like SILAC, IROA utilizes full metabolic labeling to distinguish between two mammalian cell populations but uses both 5% 13C (experimental samples) and 95% 13C (control samples) to remove artifacts/noise, calculate carbon number and molecular formula to enable metabolite identification.
News & Events
Metabolomics and Natural-Products Strategies to Study Chemical Ecology in Nematodes
Arthur S. Edison,1,* Chaevien S. Clendinen,* Ramadan Ajredini,* Chris Beecher,*,† Francesca V. Ponce* and Gregory S. Stupp‡ *Department of Biochemistry and Molecular Biology and Southeast Center for Integrated Metabolomics, University of Florida, Gainesville, FL 32610-0245, USA; †IROA Technologies, Ann Arbor, MI, USA; ‡The Scripps Research Institute, Department of Molecular and Experimental Medicine, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Integrative and Comparative Biology DOI:10.1093/ICH/ICV077. Publication Date (Web): July 2, 2015
This review provides an overview of two complementary approaches to identify biologically active compounds for studies in chemical ecology. The first is activity-guided fractionation and the second is metabolomics, particularly focusing on a new liquid chromatography-mass spectrometry-based method called isotopic ratio outlier analysis. To illustrate examples using these approaches, we review recent experiments using Caenorhabditis elegans and related free-living nematodes.