Make Profiling Easier

Mass Spectrometry Metabolite Library of Standards (MSMLSTM) supplied with software tool MSMLSDiscoveryTM

Take the hassle out of sourcing, preparing compounds and building a library!  MSMLS contains over 600 high quality primary metabolite standards provided in convenient 96 well format. The library of standards are most commonly used to provide retention times and spectra for key metabolic compounds, help optimize mass spectrometry analytical protocols, and qualify and quantify mass spectrometry sensitivity and limit of detection.  MSMLSDiscovery is a software tool designed to streamline and simplify the tedious process of building authentic standard libraries for mass spectrometry and supports the extraction, manipulation, and stoarge of the date generated when using MSMLS.

IROA Biochemical Quantitation Kit Catalog #300-250 (for Mammalian cells)

Like SILAC, IROA utilizes full metabolic labeling to distinguish between two mammalian cell populations but uses both 5% 13C (experimental samples) and 95% 13C (control samples) to remove artifacts/noise, calculate carbon number and molecular formula  to enable metabolite identification.
 

 

News & Events

New Publication!

 

Isotopic Ratio Outlier Analysis (IROA) of the S. cerevisiae metabolome using accurate mass GC-TOF/MS: A new method for discovery

Yunping Qiu 1, Robin Moir 1,  Ian M.Willis 1, Chris Beecher 2,  Yu-Hsuan Tsai 1, Timothy J. Garrett 3  Richard A. Yost  3, Irwin Jack Kurland 1
 
1 Albert Einstein College of Medicine, Bronx, NY, USA,  2 IROA Technologies LLC, Ann Arbor, MI, USA, 3 University of Florida, Gainesville, FL, USA 
 
ABSTRACT: 
Isotopic Ratio Outlier Analysis (IROA) is a 13C metabolomics profiling method that eliminates sample-to-sample variance, discriminates against noise and artifacts, and improves identification of compounds, previously done with accurate mass LC/MS. This is the first report using IROA technology in combination with accurate mass GC-TOFMS, here used to examine the S. cerevisiae metabolome. S. cerevisiae was grown in YNB media, containing randomized 95% 13C, or 5%13C glucose as the single carbon source, in order that the isotopomer pattern of all metabolites would mirror the labeled glucose. When these IROA experiments are combined, the abundance of the heavy isotopologues in the 5%13C extracts, or light isotopologues in the 95%13C extracts, follows the binomial distribution, showing mirrored peak pairs for the molecular ion. The mass difference between the 12C monoisotopic and the 13C monoisotopic equals the number of carbons in the molecules. The IROA-GC/MS protocol developed, using both Chemical and Electron Ionization, extends the information acquired from the isotopic peak patterns for formulae generation, a process that can be formulated as an algorithm, in which the number of carbons, as well as the number of methoximations and silylations, are used as search constraints. In Electron Impact (EI/IROA) spectra, the artifactual peaks are identified and easily removed, which has the potential to generate "clean" EI libraries. The combination of Chemical Ionization (CI) IROA and EI IROA affords a metabolite identification procedure that enables the identification of co-eluting metabolites, and allowed us to characterize 126 metabolites in the current study.
 
 

 

SECIM Project Awards
The University of Florida’s Southeast Center for Integrated Metabolomics (SECIM) is pleased to invite applications for its 2016 Pilot & Feasibility Project Awards, which provide up to $50,000 per award in support of 12-month metabolomics projects. Applications are due March 15, 2016.
 
BROWNBAG INFO SESSION
 
Questions or ideas? Bring them along with your lunch to SECIM’s Pilot & Feasibility RFA Info Sessions, which will be held at 12pm on Wednesday, Feb. 3, Feb. 17, Feb. 24, and Mar. 2, in Conference Room # 3161 of the Clinical and Translational Research Building.
 
PURPOSE
 
SECIM Pilot & Feasibility Project Awards seek to enhance metabolomics research by providing support for investigators new to the metabolomics field, the development of new teams and partnerships, and high-risk/ high-impact research. Successful pilot projects will provide preliminary data for new extramural grant submissions.
 
ELIGIBILITY
 
All basic, translational or clinical investigators at institutions within the United States who are eligible to apply as a principal investigator for NIH grants are eligible to apply. Young investigators and investigators who are new to the field of metabolomics are especially encouraged to apply.
 
SECIM RESOURCES
 
SECIM provides state-of-the-art metabolomics services to users in all areas of biomedical and biological sciences. Four cores provide services and develop emerging technologies for the SECIM pipeline, giving users the flexibility to choose the best approach for a particular problem:
·         Mass Spectrometry Services Core: high-throughput global and targeted metabolomics
·         Nuclear Magnetic Resonance Core: global metabolomics and biomarker identification
·         Advanced Mass Spectrometry Core: biomarker identification, imaging mass spectrometry and isotopic ratio outlier analysis
·         Bioinformatics Core: quality controlled data from the three analytical cores will be available to users with several statistical analysis options in an interactive web interface
 
TIMELINE
·         Application Deadline: 5 p.m. on Tuesday, March 15, 2016
·         Earliest Award Start Date: June 15, 2016
 
AWARD AMOUNTS & BUDGET DEVELOPMENT
 
The one-year awards support up to $50,000 per award in SECIM services, with preference given to applications with budgets of $35,000 or less to enable the funding of as many unique and innovative projects as possible. Requests for salary for the principal investigator or personnel, laboratory costs or extramural services outside of SECIM are not permissible. All funds will be awarded in credit toward SECIM services, or in payment for the project’s materials and supplies. Applicants are encouraged to consult with SECIM cores to develop budget estimates.
 
RFA AND APPLICATION INSTRUCTIONS
 
For complete award details and requirements, download the RFA and application instructions:
http://bit.ly/1mafvAs
 
CONTACT FOR CONSULTS & QUESTIONS
 
Email SECIM to request a consultation with the core(s) of interest, or with any questions not addressed in the RFA or application instructions: info@secim.ufl.edu