In addition to promising a program full of research topics such as metabolomics and metabolic cancer research, this international meeting will cover sessions on proteomics, genomics, and NMR and Mass Spectrometric analytical and isotopic techniques including new advanced technologies.
Generating Accurate Metabolomics Data using the IROA Protocol
November 6th 2 PM EST
This tutorial demonstrated the IROA (Isotopic Ratio Outlier Analysis) protocol.
Step 1: 5% and 95% 13C-labeled cell culture media achieves full metabolic labeling of experimental and control samples, respectively: a) Internal Standards are generated for every metabolite measured; b) two or more cell populations are readily distinguished; and 3) artifacts and noise are readily distinguished from biological compounds. Step 2: ClusterFinder™ software performs accurate biochemical quantitation: a) characterizes peaks (artifacts, control, experimental); b) removes artifacts and noise; c) calculates carbon number and MF, d) quantitates control/experimental ratios; and e) normalizes datasets. Step 3: IROA's Portal tools execute data interpretation and statistical analysis.