1. IROA-LTRS
Long-Term Reference Standard
- Paired 1:1 mixture of labeled U-5% and U-13C metabolites
- Analyzed every 10 samples for QC
- Builds triply redundant dictionary
TruQuant Yeast Extract Semi-targeted QC Workflow Kit
Made from unique fully-labeled S. cerevisiae yeast extract containing 100s of polar primary metabolites and spanning a broad range of metabolic classes.
- Semi-targeted, LC-MS-based analysis workflow
- Ion suppression correction and "Dual MSTUS" sample normalization
- Accurate metabolite ID and quantitation
- Applicable to numerous sample matrices
500+
Polar Metabolites
Unique metabolites in yeast extract
90
Samples Per Kit
Economical solution for high-throughput labs
$200
Per 90 Samples
Cost-effective metabolomics QC workflow
3-Step
Workflow
Simple, robust and reproducible process
Vendor
Agnostic
Compatible with major LC-MS platforms
Why TruQuant?
Traditional metabolomics workflows suffer from ion suppression, retention time drift, and poor sample normalization.
TruQuant introduces a dual-standard approach using:
IROA-LTRS (Long-Term Reference Standard)
For system QC and dictionary generation
IROA-IS (Internal Standard)
For accurate quantitation and normalization
Together, they deliver accurate, reproducible, and comparable metabolomics data across experiments, instruments, and laboratories.
How the TruQuant Workflow Works
1
Analyze Samples
Run experimental samples with IROA-IS. Inject IROA-LTRS every 10 samples as QC Standard to account for fluctuations in mass and retention time drift.
2
Build Metabolite Dictionary
ClusterFinder software generates a "dictionary" of RT, m/z, formula and physical characteristics from the analysis of IROA-LTRS.
3
Identify & Quantitate
Dictionary and IROA-IS enable compound identification, suppression correction, normalization, and improved metabolomics data accuracy.
What's Included
2. IROA-IS
Internal Standard
- Labeled 95% U-13C metabolites
- Spike into samples for quantitation
- Yardstick for accurate measurements
3. ClusterFinder Software
Powerful Analysis Engine
- Generates metabolite libraries
- Identifies and quantifies compounds
- Corrects ion suppression and normalizes data
4. TruQuant User Manual
Comprehensive Guide
- Step-by-step instructions
- Workflow setup and best practices
- Troubleshooting and FAQs
Summary of Benefits
Daily instrument QA/QC
Economical $200 for 90 samples
No false data; removal of artifactual peaks
Accurate compound formula ID for MS alone
Corrects for ionization losses for better quantitation
Normalizes samples to a universal standard
Yeast metabolites characterized by multiple LC-MS methods
ClusterFinder builds libraries, IDs and quantitates
Mass-spec vendor agnostic support
Supports biomarker discovery
Helpful Resources
Routinely Identified Compounds in Internal Standard
DownloadReproducibility of Metabolomic Profiles (Round Robin Study)
DownloadPoster – Correction of Ion Suppression and Normalisation
DownloadWhitepaper – Integration of Standards for Ion Suppression
DownloadHow to Set Up and Run a C13-labelled Yeast Extract Calibration
DownloadReady to Improve Quantitative Accuracy?
TruQuant delivers reliable identification, ion suppression correction and normalization to elevate your metabolomics research.