TruQuant Yeast Extract Semi-targeted QC Workflow Kit

 

The Workflow is a high-quality quantitation system that utilizes two (2) complex Internal Standards to make accurate measurements of several hundred metabolites simultaneously.
  • Semi-targeted, LC-MS-based analysis Workflow
  • Ion suppression correction and “Dual MSTUS” sample normalization
  • Accurate metabolite ID and quantitation
  • Applicable to numerous sample matrices
  • Daily method and instrument QC
  • Biomarker discovery
Materials
Made from unique fully-labeled S. Cerevisiae Yeast Extract containing 100s of polar primary metabolites and spanning a broad range of metabolic classes.

  1. IROA LONG-TERM REFERENCE STANDARD – IROA-LTRS (paired 1:1 mixture of labeled U-5% and – 95% U-13C metabolites): The LTRS is analyzed every 10 samples for QC and used to build a triply redundant dictionary (library) of RT, m/z and physical characteristics using ClusterFinder software.
  2. IROA INTERNAL STANDARD – IROA-IS (labeled 95% U-13C metabolites): – spike into experimental samples and use as a yardstick in which to QUANTIFY all biochemicals in the sample relative to their counterparts in the IROA-LTRS. Even if analyzed on a different chromatographic system, results can be equated using dictionary because of the IROA-IS. Experimental compounds may have different RTs but will exhibit the same mass and physical characteristics.
  3. CLUSTERFINDER SOFTWARE
  4. TruQuant USER MANUAL

TruQuant Yeast Extract Semi-targeted QC Workflow 3 step process

  1. LC-MS analysis of experimental samples with IROA-IS.  Inject IROA-LTRS periodically i.e. 10 sample intervals, as QC Standard to account for fluctuations in mass and retention time drift.
  2. Generate “dictionary” of RT, m/z, formula and physical characteristics from the analysis of IROA-LTRS using ClusterFinder software.
  3. ClusterFinder software and dictionary identifies/quantitates, suppression corrects and normalizes compounds in experimental samples.

Summary of Benefits of the Yeast Extract Semi-targeted Workflow

  1. Daily instrument QA/QC.
  2. Economical – $200 for 90 samples
  3. No false data; removal of artifactual peaks.
  4. Accurate compound formula ID for MS alone; complete ID with addition of SWATH, or IM.
  5. Corrects for all source ionization losses for significantly better quantitation.
  6. Normalizes samples to a universal standard for complete comparability.
  7. Yeast metabolites have been characterized by several LC-MS methods and incorporated into ClusterFinder software.
  8. ClusterFinder software solution builds libraries, IDs/quantitates compounds, corrects for ion-suppression and normalizes data.
  9. Mass-spec vendor agnostic; specific extensions for inclusion of Sciex SWATH, and Agilent IM.

Helpful resources

ROUTINELY IDENTIFIED COMPOUNDS IN INTERNAL STANDARD

REPRODUCIBILITY OF METABOLOMIC PROFILES GENERATED USING THE TRUQUANT PLATFORM IN A MULTI-LAB “ROUND ROBIN” STUDY DESIGN

POSTER – Correction of Ion Suppression and Normalization for Improved Quantitative Rigor and Reproducibility using IROA

WHITEPAPER – Integration of Standards for Ion Suppression Correction and QC in an Untargeted Metabolomics Workflow

How to set up and run a C13-labeled Yeast Extract “TruQuant” Calibration Experiment

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