IROA utilizes full metabolic labeling to embed mathematically calculable signatures into biochemical metabolites and uses software algorithms to find, identify and quantitate these specific peaks.

How is this achieved?
  Compounds are randomly and universally labeled and the relative abundances of the isotopes of carbon are altered; enriched in one particular isotope and depleted in its other isotopic form. For example,  95% U-13C6-Glucose is not actually 95% 13C6 and 5% 12C6. Instead each carbon atom position (1,2,3,4,5,6) has a 95% chance of being 13C labeled and a 5% chance of being 12C. Compounds such as 5% and 95% U-13C6-Glucose are produced especially for IROA Technologies LLC by Cambridge Isotope Technologies, Inc.
IROA Benefits

The IROA ClusterFinder™ software tool characterizes peaks (as either artifacts, control or experimental compounds), removes artifacts, performs peak correlation analysis to associate adducts with their fragments, calculates carbon number and molecular formula, quantitates the ratio of control to experimental, corrects for ion-suppression and normalizes each dataset.